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LncRNA AGAP2-AS1 interacts with IGF2BP2 to promote bladder cancer progression via regulating LRG1 mRNA stability 1 1 Introduction 1 2 Materials and methods 2 2.1 Patients and tissue samples 2 2.2 Cell cultures 2 2.3 Cell transfection 2 2.4 Extraction of cytoplasmic and nuclear RNAs 2 2.5 RT-qPCR 3 2.6 Western blot 3 2.7 Cell proliferation assay 3 2.8 Colony formation assay 3 2.9 Transwell assay 3 2.10 Tube formation assay 3 2.11 Actinomycin D test 3 2.12 RNA immunoprecipitation (RIP) assay 3 2.13 RNA pull-down assay 3 2.14 Animal experiments 3 2.15 Immunostaining assay 4 2.16 Fluorescence in situ hybridization (FISH) 4 2.17 Statistical analysis 4 3 Results 4 3.1 The upregulation of AGAP2-AS1 was closely associated with a poor prognosis of BCa 4 3.2 AGAP2-AS1 promoted migration, invasion, and angiogenesis of BCa cells 4 3.3 AGAP2-AS1 could directly interact with IGF2BP2 4 3.4 AGAP2-AS1 enhanced the stability of LRG1 mRNA by recruiting IGF2BP2 7 3.5 AGAP2-AS1 downregulation inhibited the migration, invasion and angiogenesis of BCa cells by inhibiting LRG1 9 3.6 AGAP2-AS1 silencing inhibited tumor growth and angiogenesis in vivo 10 4 Discussions 11 Conclutions 11 Ethics approval and c

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nonfiction