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GHS-R1a deficiency mitigates lipopolysaccharide-induced lung injury in mice via the downregulation of macrophage activity 1 1. Introduction 1 2. Materials and methods 2 2.1. Animals 2 2.2. LPS-induced lung injury 2 2.3. Plasma ghrelin determination 2 2.4. Histology 2 2.5. Murine bronchoalveolar lavage (BAL) experiment 2 2.6. Measurement of arterial oxygen saturation 2 2.7. Quantitative real-time PCR 2 2.8. Western blotting 2 2.9. Enzyme-linked immunosorbent assay (ELISA) 2 2.10. Isolation of mouse peritoneal macrophage 2 2.11. Mitochondrial respiration assays 2 2.12. Statistical analyses 2 3. Results 3 3.1. Ghsr−/− mice survived after LPS-induced lung injury 3 3.2. GHS-R1a deficiency ameliorated the lung inflammation and disruption of alveolar barrier after injury 3 3.3. Muted firing of inflammatory responses in ghsr−/− mice after lung injury 3 3.4. GHS-R1a deficiency lowers LPS-induced mitochondrial respiration and induction of inflammatory cytokines in macrophages 4 4. Discussion 4 Declaration of competing interest 6 Acknowledgments 6 References 6
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