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Ampelopsin attenuates Staphylococcus aureus Alpha-Toxin-Induced Lung Injury 1 1 Introduction 1 2 Material and methods 2 2.1 Bacteria and reagents 2 2.2 Mice and in vivo inoculation 2 2.3 Pharmacological interventions and survival curves 2 2.4 Bacterial load of lung tissue 2 2.5 Pulmonary mechanics study 3 2.6 Pathological histology 3 2.7 Total protein leakage in alveolar lavage fluid 3 2.8 Determination of cytokine production by ELISA 3 2.9 Protein immunoblotting for ADAM10 and E-cadherin expression 3 2.10 Cell damage assay and inflammatory protein assay 3 2.11 Statistics 3 3 Results 3 3.1 Ampelopsin alleviates alpha-toxin-induced animal mortality during S. aureus infection 3 3.2 Ampelopsin prevents S. aureus from colonizing mouse lungs 4 3.3 Ampelopsin increases respiratory function in mice with bacterial lung damage 4 3.4 Ampelopsin improves infectious lung injury-induced tissue damage in mice 4 3.5 Ampelopsin attenuates total protein leakage from alveolar lavage fluid in mice 4 3.6 Ampelopsin decreases the release of pro-inflammatory mediators in mouse alveolar lavage fluid 4 3.7 Western blot assay for ADAM10 and E-cadherin 4 3.8 Effect of ampelopsin on the expression of NF-κB/N

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UND

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nonfiction