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Berberine suppresses cecal ligation and puncture induced intestinal injury by enhancing Treg cell function 1 1 Introduction 1 2 Materials and methods 2 2.1 Mice 2 2.2 LPS and CLP-induced sepsis models 2 2.3 Histological analysis 2 2.4 Quantitative real-time PCR 2 2.5 Serum endotoxin level measurement 2 2.6 Protein isolation and Western blot analysis 2 2.7 Flow cytometry 2 2.8 Cytokine analysis 3 2.9 In vitro macrophage suppression assay 3 3 Statistical analysis 3 4 Results 3 4.1 Effect of BBR pretreatment on the mortality of septic mice 3 4.2 BBR alleviates intestinal injury and reduces serum endotoxin level 4 4.3 BBR protects the lung and liver from apoptosis during sepsis 4 4.4 Effect of BBR on immune cells (mesenteric lymph node cells, MLN cells) following CLP-induction 5 4.5 Depletion of Treg cells leads to severe intestinal injury and higher pro-inflammatory cytokines 5 4.6 Treg cells of BBR pretreated mice restrain LPS-induced inflammatory response in macrophages 5 5 Discussion 5 CRediT authorship contribution statement 10 Declaration of Competing Interest 10 Acknowledgements 10 Appendix A Supplementary data 10 References 10

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UND

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nonfiction