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Fractionating process of lignocellulosic biomass for the enzymatic production of short chain cello-oligosaccharides 1 1 Introduction 1 2 Material and methods 2 2.1 Raw sample 2 2.2 Cellulose extraction process 2 2.2.1 Treatments severity factor 2 2.2.2 Delignification process 3 2.2.3 Alkaline treatments 3 2.2.4 Solid recovery yield and cellulose extraction efficiency 3 2.3 Chemical composition determination 3 2.4 Enzymes and determination of protein concentrate 3 2.5 Enzyme activity measurements 3 2.6 Enzymatic hydrolysis and experimental design 3 2.7 Glucose and cello-oligosaccharides quantification 3 2.8 Statistical analysis 3 3 Results and discussion 4 3.1 Cellulose extraction 4 3.1.1 Chemical characterization 4 3.1.2 Solid recovery and cellulose extraction efficiency 4 3.1.3 Extracted cellulose as a substrate for cellulase enzymatic activity 5 3.2 Cello-oligosaccharides production 5 4 Conclusion 7 CRediT authorship contribution statement 7 Declaration of Competing Interest 7 Acknowledgments 8 Appendix A Supporting information 8 References 8

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Language

UND

Category

nonfiction