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Resolvin D2–G-Protein Coupled Receptor 18 Enhances Bone Marrow Function and Limits Steatosis and Hepatic Collagen Accumulat ... 1 Materials and Methods 2 Experimental Animals 2 Generation of a Humanized GPR18 Floxed Mouse 2 RvD2 Treatment 2 Complete Blood Cell Count 3 RNA Sequencing 3 Liver Picrosirous Red Staining 3 Liver Immunofluorescence 3 Oil Red O Staining 4 Triglyceride Assay 4 Human Liver Autopsy Specimen 4 Tissue Processing and Flow Cytometry 4 Liver qPCR 5 RNAscope 5 Colony-Forming Unit Assays 5 Bone Marrow Transplantation Experiments 6 Peritoneal Macrophage Immunofluorescence (in Vitro) 6 C-Reactive Protein, Insulin, and Col-IV Enzyme-Linked Immunosorbent Assays 6 Phagocytosis Assay 6 Statistical Analysis 6 Results 6 Gpr18 Expression Is Increased in Macrophages from Old Mice and in Livers from Old Humans 6 Middle-Aged Mice Have Increased Liver Collagen and Fatty Deposits 7 Myeloid Gpr18 Limits Fat and Collagen Accumulation in Livers of Aging Mice 9 Resolvin D2 Limits Age-Related Steatosis and Collagen Accumulation in the Liver 10 RvD2 Increases Monocytes in Bone Marrow and Promotes Monopoiesis 11 RvD2 Limits Age-Related Collagen Accumulation in the Liver in Part through

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