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Increased expression of sodium-glucose cotransporter 2 and O-GlcNAcylation in hepatocytes drives non-alcoholic steatohepatitis 1 1 Introduction 2 2 Materials and methods 2 2.1 Human liver tissue 2 2.2 Animals 2 2.3 Histological analysis 2 2.3.1 Immunohistochemistry 2 2.3.2 Hematoxylin and eosin (H&E), trichrome, sirius red staining 3 2.4 Hepatic triglyceride 3 2.5 Blood chemistry assays in in vivo models 3 2.6 Non-invasive assessment of hepatic steatosis and fibrosis in human subjects 3 2.7 In vitro cell culture system 3 2.7.1 Cell culture 3 2.7.2 Oil red O staining 3 2.7.3 In vitro study models 3 2.8 Immunoblot analysis and antibodies 4 2.9 Quantitative RT-PCR analysis 4 2.10 Statistics 4 3 Results 4 3.1 Human NASH demonstrates increased SGLT2 expression and O-GlcNAcylation in liver 4 3.2 Upregulated SGLT2 expression in association with intracellular O-GlcNAcylation in hepatocytes under NASH conditions and ... 4 3.3 Decreased expression of SGLT2 attenuates O-GlcNAcylation-mediated inflammation in hepatocytes under NASH conditions 5 3.4 SGLT2 inhibitor induces autophagic flux through AMPK-TFEB activation via reduction of intracellular O-GlcNAcylation 7 3.5 SGLT2 inhibitor ameliorat

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UND

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nonfiction