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Nose to brain delivery of donepezil through surface modified NLCs: Formulation development, optimization, and brain targeti ... 1 1 Introduction 1 2 Materials and method 2 2.1 Materials 2 2.2 Methods 3 2.2.1 Preliminary screening study 3 2.2.2 Optimization of DPZ-CH-NLCs by Box-Behnken design 4 2.2.3 Preparation of DPZ-loaded NLCs 4 2.2.4 Surface modification of DPZ-NLCs by CH 4 2.2.5 Characterization of DPZ-CH-NLCs 4 2.2.5.1 Particle size, zeta potential, polydispersity index, and structural evaluation 4 2.2.5.2 Determination of DPZ entrapment efficiency 4 2.2.5.3 Thermal study by DSC 4 2.2.5.4 In-vitro drug release 4 2.2.5.5 Ex-vivo permeation study 4 2.2.5.6 In-situ nasal perfusion/absorption 4 2.2.5.7 Mucoadhesion study 5 2.2.6 In-vivo study 5 2.2.6.1 Dose calculation, animals grouping, and drug administration 5 2.2.6.2 Method of blood collection 5 2.2.6.3 Extraction of DPZ from collected blood 5 2.2.6.4 Isolation, collection, and extraction of brain 5 2.2.6.5 HPLC specification and mobile phase 5 2.2.6.6 Detection of pharmacokinetic and neuropharmacokinetic parameters 5 2.2.7 Stability study 6 2.2.8 Statistical evaluation 6 3 Results and discussion 6 3.1 Preliminary screening

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nonfiction