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Box-Behnken design optimized silibinin loaded glycerylmonooleate nanoliquid crystal for brain targeting 1 1 Introduction 1 2 Materials 2 2.1 Chemicals 2 3 Method of NLCs preparation 2 4 SIL-NLCs BBD optimization 2 5 Characterization of NLCs 2 5.1 Percentage drug loading 2 5.2 Particle size analysis 3 5.3 Fourier transform infrared spectroscopy (FTIR) 3 5.4 Differential scanning calorimetry (DSC) 3 5.5 Transmission electron microscopy (TEM) 3 5.6 Stability of NLCs in simulated biological fluids 3 5.7 In vitro drug release study 3 6 Pharmacokinetic studies 3 6.1 Liquid chromatography-mass spectroscopy (LCMS) method for SIL analysis 3 6.2 Oral bioavailability study 3 6.3 Silibinin brain uptake and biodistribution quantification 3 6.4 Statistical analysis 4 7 Results and discussion 5 7.1 Preparation of SIL-NLCs and optimization 5 7.2 Optimization SIL-NLCs on BBD 5 7.2.1 Particle size 6 7.2.2 % DL 6 7.2.3 Data optimization and model validation 6 7.3 FTIR 7 7.4 DSC 7 7.5 TEM 8 7.6 Stability studies in biological media 8 7.7 In-vitro cumulative drug release 8 8 Pharmacokinetic studies 10 8.1 Determination of silibinin concentration in rat plasma using LCMS 10 8.2 Oral bioavailability of S

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nonfiction