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Triiodothyronine attenuates neurocognitive dysfunction induced by sevoflurane in the developing brain of neonatal rats 1 1 Introduction 1 2 Methods 2 2.1 Animals and chemicals 2 2.2 Sevoflurane exposure and T3 treatment 2 2.3 Behavioral procedures 2 2.3.1 Morris water maze (MWM) and open field test (OFT) 2 2.3.2 Histology and immunohistochemistry 2 2.3.3 Nissl staining 3 2.3.4 Bromodeoxyuridine (BrdU) staining 3 2.3.5 Golgi staining 3 2.3.6 Western blot 3 2.3.7 Statistical analysis 3 3 Results 3 3.1 Sevoflurane-impaired cognitive function was improved by T3 treatment 3 3.2 Sevoflurane caused cell death and inhibited neural proliferation which was prevented by T3 treatment 3 3.3 Sevoflurane-suppressed neurogenesis in CA3 (but not in CA1) of the hippocampus in rats at P30 was subsequently promoted ... 3 3.4 NR2A and NR2B NMDA receptor subunits expression and PSD-95 in the hippocampus at P15 inhibited by sevoflurane exposure ... 4 4 Discussion 4 5 Conclusion 7 Declaration of Competing Interest 7 Funding 7 CRediT authorship contribution statement 7 Acknowledgments 7 References 7

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nonfiction