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Role of simulated in vitro gastrointestinal digestion on biotransformation and bioactivity of astragalosides from Radix Ast ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials and chemicals 2 2.2 In vitro digestion 2 2.3 Ultrahigh-performance liquid chromatography quadrupole time-of-flight-mass spectrometry (UPLC-Q-TOF/MS) analysis 3 2.4 Method validation 3 2.5 Cell culture, cytotoxicity and cytosolic and mitochondrial oxidative stress 3 2.6 Statistical analysis 3 3 Results and discussion 3 3.1 Quantification of the main constituents in the extract and digestive products of RA 3 3.2 Transformation of astragalosides based on deacetylation and transacetylation 4 3.3 Cellular antioxidant activity of RA extract and its digestive active ingredients 4 4 Conclusion 7 CRediT authorship contribution statement 7 Declaration of Competing Interest 7 Acknowledgement 7 Appendix A Supporting information 7 References 7
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