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Nrf2 protects against methamphetamine-induced nephrotoxicity by mitigating oxidative stress and autophagy in mice 1 1 Introduction 1 2 Materials and methods 2 2.1 Reagents 2 2.2 Animal models 2 2.3 Serum assays 2 2.4 Measurement of malondialdehyde (MDA) and superoxide dismutase (SOD) 2 2.5 Hematoxylin and eosin (H&E) staining 2 2.6 Immunohistochemical staining and immunofluorescence staining 2 2.7 Western blotting 3 2.8 Statistical analysis 3 3 Results 3 3.1 MA-induced renal damage, oxidative stress, and autophagy 3 3.2 MA activated Nrf2/HO-1 signaling pathway in kidney 3 3.3 Activation of Nrf2 may mitigate MA-induced renal damage, oxidative stress, and autophagy 3 3.4 Knockout of Nrf2 may aggravate MA-induced renal damage, oxidative stress, and autophagy 3 4 Discussion 5 Funding 12 Declaration of Competing Interest 12 Appendix A Supporting information 12 References 12

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UND

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nonfiction