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Oxidase-mimicking peptide-copper complexes and their applications in sandwich affinity biosensors 1 1 Introduction 2 2 Experimental section 2 2.1 Materials and reagents 2 2.2 Modification of AuNPs 2 2.3 Preparation of sensor electrodes 3 2.4 Voltammetric characterization of peptide-Cu2+ 3 2.5 Mass spectra 3 2.6 Absorption measurement of AA 3 2.7 Electrochemical detection of PSA 3 2.8 Fluorescent assays of PSA 3 3 Results and discussion 3 3.1 Redox behaviors of peptide-Cu(II) complexes 3 3.2 Catalytic oxidation of AA by peptide-Cu2+ complexes 4 3.3 Principle of the biosensors 5 3.4 Feasibility of the methods 5 3.5 Sensitivity for PSA detection 6 3.6 Selectivity and real sample assays 7 4 Conclusion 7 CRediT authorship contribution statement 7 Declaration of competing interest 7 Acknowledgments 7 Appendix A Supplementary data 7 References 7
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