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Dysregulation of ferroptosis may participate in the mitigating effect of CoCl2 on contrast-induced nephropathy 1 Introduction 2 Materials and methods 3 Animals and treatments 3 Enzyme-linked immunosorbent assay (ELISA) 4 Hematoxylin and eosin (H&E) staining and PAS (periodic acid-Schiff) staining 4 Proteomics 4 Bioinformatics analysis 4 Cell culture and treatment 4 Transfection 5 Cell viability assay 5 Iron quantification 5 Analysis of the production of reactive oxygen species (ROS) 5 Determination of ferroptosis by measuring lipid peroxides 5 RNA isolation and real-time PCR 5 Western blotting analysis 5 Immunofluorescence staining 5 Statistical analysis 6 Results 6 CoCl2 decreased renal function indicators after CIN 6 CoCl2 attenuated the renal tubular damage caused by CIN 6 Quantitative proteomic analysis of kidney tissues from CIN mice receiving CoCl2 treatment 6 The expression levels of Hp decreased in CIN mice after treatment with CoCl2 6 CoCl2 plays a protective role in CIN by inhibiting ferroptosis by upregulating Hp in vitro 6 Discussion 8 Conclusion 13 Funding 13 Conflicts of interest 13 Acknowledgement 13 Appendix A Supplementary data 13 References 13

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Language

UND

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nonfiction