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Metformin treatment ameliorates endocrine-metabolic disturbances in letrozole-induced PCOS mice model by modulating adipone ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Chemicals 2 2.2 Experimental animal 2 2.3 Confirmation of PCOS induction 3 2.4 Biochemical analysis 3 2.4.1 Serum hormone analysis 3 2.4.2 Serum fasting glucose 3 2.4.3 Serum lipid profile 3 2.4.4 Antioxidant assay 3 2.4.4.1 Lipid peroxidation assay 3 2.4.4.2 Reduced glutathione assay 3 2.4.4.3 Superoxide dismutase 3 2.5 Plasma adiponectin 3 2.6 Pro-inflammatory/inflammatory biomarkers (interleukin-6) 3 2.7 Histological assessment of ovaries 3 2.8 Statistical analysis 3 3 Results 5 3.1 Effects of different treatments on body weight 5 3.2 Effect of metformin on serum hormonal levels 5 3.3 Effect metformin treatment on glucose, cholesterol and triglycerides (TG) levels in PCOS mice 6 3.4 Effect of metformin on antioxidant activity and lipid peroxidation 6 3.5 Metformin administration treatment reduces plasma IL-6 in rats with letrozole induced PCOS 6 3.6 Metformin treatment increases circulating adiponectin concentration in mice with letrozole induced PCOS 6 4 Discussion 6 5 Conclusion 8 Funding 9 Data availab

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Language

UND

Category

nonfiction