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Quantitation Of Mrna By Polymerase Chain Reaction: Nonradioactive Pcr Methods (springer Lab Manuals) by TH.KOHLER D.LABNER A.-K.ROST B.THAMM B.PUSTOWOIT H.REMKE, Thomas Köhler, Dirk Laßner, Anne-Katrin Rost, Barbara Thamm, Barbara Pustowoit, Harald Remke, Thomas Köhler, Dirk Lassner, Barbara Thamm, Barbara Pustowoit, Th. Köhler ... [et al.] is a book available to read on EtoBox.
What is Quantitation Of Mrna By Polymerase Chain Reaction: Nonradioactive Pcr Methods (springer Lab Manuals) about?
In this laboratory \"cook-book\", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e. g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; nRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research. In this laboratoy "cook-book", the authors provide a concise guide to PCR-based quantification of nucleic acids using exclusively nonradioactive detection. The procedures described are presented in a detailed and practice-oriented manner to enable professionals and beginners to adopt easily quantitative PCR protocols into their own research.
- Author
- TH.KOHLER D.LABNER A.-K.ROST B.THAMM B.PUSTOWOIT H.REMKE, Thomas Köhler, Dirk Laßner, Anne-Katrin Rost, Barbara Thamm, Barbara Pustowoit, Harald Remke, Thomas Köhler, Dirk Lassner, Barbara Thamm, Barbara Pustowoit, Th. Köhler ... [et al.]
- Publisher
- Springer Spektrum. in Springer-Verlag GmbH
- Published
- 1995
- Language
- EN
- ISBN
- 9783540591924
- Subjects
- Science, Chemistry, Medical
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