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Basic Cloning Procedures (springer Lab Manuals) by Valdis Berzins (ed.) is a book available to read on EtoBox.
What is Basic Cloning Procedures (springer Lab Manuals) about?
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
- Author
- Valdis Berzins (ed.)
- Publisher
- Springer Spektrum. in Springer-Verlag GmbH
- Published
- 2012
- Language
- EN
- ISBN
- 9783642719653
- Subjects
- Science, Chemistry, Biology
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