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Electrochemical sensing of doxorubicin hydrochloride under sodium alginate antifouling conditions using silver nanoparticle ... 1 1 Introduction 1 2 Experimental 2 2.1 Chemicals and reagents 2 2.2 Apparatus and measurements 2 2.3 Sensor design 2 2.3.1 Electrode pretreatment and cleaning 2 2.3.2 Synthesis of AgNPs on the surface of GCE by electrodeposition technique 2 2.3.3 Electrodeposition of Alg on the surface of AgNPs modified GCE 2 2.4 Cell culture and biological tests 2 2.4.1 General cell culture procedure 2 2.4.2 Fluorescence imaging of the cell uptake of DOX 2 2.4.3 Determination of DOX in cell lysates 2 3 Results and discussions 2 3.1 Electrode modification and characterization of AgNPs and Alg modified GCE 2 3.2 Electrochemical behavior of poly-HAlg/AgNPs/GCE platform towards DOX 4 3.3 Effect of pH 4 3.4 Kinetic study of the oxidation of DOX 4 3.5 Analytical performance 6 3.5.1 Calibration curve 6 3.6 Method validation 7 3.6.1 Accuracy, inter-day and intra-day repeatability 7 3.6.2 Specific manner of the developed method toward DOX detection 7 3.7 Applications of the AgNPs/p-Alg modified GCE probe 7 3.7.1 Detection of DOX in cell lysates samples 7 3.7.2 Detection of DOX in

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UND

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nonfiction