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Identifications of metabolic differences between Hedysari Radix Praeparata Cum Melle and Astragali Radix Praeparata Cum Mel ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Experimental herbs 2 2.2 Preparation, chemical compositions and quality analysis of HRPCM and ARPCM 2 2.3 Animals 2 2.4 Experimental protocols and Drug intervention 2 2.5 Sample collection 2 2.6 H&E staining pathological analysis 3 2.7 Enzyme-linked immunosorbent assay 3 2.8 Metabolomics analysis 3 2.9 Statistical methods 3 3 Results 3 3.1 Qualitative analysis of HRPCM and ARPCM 3 3.2 The Effect of HRPCM and ARPCM on SQD rats 3 3.3 Metabolomics analysis 3 3.3.1 HRPCM and ARPCM treatments regulated metabolic profiling of serum and spleen in SQD rats 3 3.3.2 Comparison of the levels of the common differential metabolites in serum and spleen amongst groups 4 3.3.3 Screening of differential metabolites and pathways in serum and spleen between HRPCM and ARPCM for SQD rats 4 3.4 Identification of potential biomarkers 5 3.4.1 Identification of potential biomarkers of ARPCM for SQD rats 5 3.4.2 Identification of potential biomarkers of HRPCM for SQD rats 5 3.5 Differences of biomarkers between HRPCM and ARPCM for SQ

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nonfiction