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Characterisation of products from EDC-mediated PEG substitution of chitosan allows optimisation of reaction conditions 1 1 Introduction 1 2 Experimental 2 2.1 Materials 2 2.2 Synthesis of PEGylated CHI and EDC-CHI 2 2.3 Characterisation 2 2.3.1 Fourier transform infrared (FTIR) spectroscopy 2 2.3.2 Nuclear magnetic resonance (NMR) spectroscopy 2 2.3.3 Elemental analysis 2 2.3.4 X-ray photoelectron spectroscopy (XPS) 2 2.4 Solution properties 3 2.5 Cell cytotoxicity 3 2.6 Statistical analysis 3 3 Results and discussion 3 3.1 Chemical characterisation 3 3.2 Effect of reactant ratios on the PEGylation reaction 5 3.3 Solution properties 6 3.4 Cytotoxicity analysis 7 4 Conclusion 7 CRediT authorship contribution statement 7 Declaration of competing interest 7 Acknowledgements 8 Appendix A Supplementary data 8 References 8
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