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Preparation, characterization and antioxidant properties of curcumin encapsulated chitosan/lignosulfonate micelles 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials and reagents 2 2.2 Preparation of chitosan/lignosulfonate micelles and preparation of curcumin encapsulated chitosan/lignosulfonate micelles 2 2.3 Determination of critical micelle concentration (CMC) 2 2.4 Encapsulation efficiency of curcumin 2 2.5 Determination of particle size, PDI and potential 3 2.6 Structural characterization of micelles 3 2.6.1 Morphological characterization 3 2.6.2 Solid state NMR carbon spectroscopy 3 2.6.3 Fourier transform infrared (FTIR) spectroscopy 3 2.6.4 UV–visible spectroscopy analysis 3 2.6.5 X-ray diffraction analysis (XRD) 3 2.6.6 Differential scanning calorimetry (DSC) analysis 3 2.7 Stability assays of micelles 3 2.7.1 Storage stability assay 3 2.7.2 pH stability assay 3 2.7.3 Thermal stability assay 3 2.8 Release curve of curcumin 3 2.9 Determination of antioxidant activity 3 2.9.1 DPPH assay 3 2.9.2 ABTS assay 3 2.10 Statistical analysis 4 3 Results and discussion 4 3.1 Critical micelle concentration (CMC) of CS-LS and encapsulation efficiency of curcumin 4 3.2 Determina
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