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Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant 1 1 Introduction 1 2 Material and methods 2 2.1 Plant material 2 2.2 Media preparation and sterilization 2 2.3 Sterilization of seeds 2 2.4 Inoculation and incubation 2 2.5 Callus induction and regeneration 2 2.6 Direct regeneration 3 2.7 Rooting 3 2.8 Acclimatisation 3 2.9 Statistical analysis 3 3 Results 3 3.1 Callus induction 3 3.2 Indirect shoot regeneration and plantlet formation 4 3.3 Direct regeneration 4 3.4 Root induction 5 4 Discussion 6 5 Conclusion 7 Contributions 7 Declaration of Competing Interest 7 Acknowledgements 7 References 7
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