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Submergence promotes auxin-induced callus formation through ethylene-mediated post-transcriptional control of auxin receptors 1 Introduction 1 Results 2 Submergence promotes callus formation 2 Submergence changes auxin responses 5 Ethylene controls callus formation 5 Post-transcriptional regulation of auxin receptors by ethylene 5 Regeneration capacity of submergence-induced calli 10 Discussion 10 Methods 11 Plant materials 11 Plasmid construction 11 Agrobacterium-mediated transformation of Arabidopsis 12 Plant growth conditions 12 Incubation of leaf explants for callus formation 12 Pharmacological treatment 12 Visualizing callus formation patterns 12 RNA extraction and gene expression analysis by quantitative PCR 12 Thin sectioning and imaging 12 Stem-loop qRT–PCR 12 GUS staining 13 Fluorescence imaging 13 RNA-seq 13 In vitro ribonuclease activity assay 13 Statistical analysis 13 Data availability 13 Accession numbers 13 Supplemental information 13 Acknowledgments 13 References 13
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Common subject areas: history, science, philosophy, social sciences.
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