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Differential expression profiles and function prediction of tRNA-derived fragments in fibrous dysplasia 1 1 Background 1 2 Materials and methods 2 2.1 Primary cell culture 2 2.2 RNA extraction and quality control 2 2.3 Library preparation and high-throughput sequencing 3 2.4 Analysis of high-throughput sequencing data 3 2.5 Real-time PCR and statistics 3 2.6 Target gene prediction of tRFs/tiRNAs 3 2.7 GO, KEGG, and PPI network analyses 3 2.8 Statistical analysis 4 3 Results 4 3.1 Expression profiles of tRFs/tiRNAs in FD BMSCs and normal BMSCs 4 3.2 Differential expression of tRFs/tiRNAs in FD BMSCs and BMSCs 4 3.3 Validation of differential expression tRFs/tiRNAs in BMSCs and FD BMSCs 4 3.4 Prediction of target genes with bioinformatics tool 4 3.5 GO enrichment and KEGG pathway analysis 4 3.6 PPI network analyses 5 4 Discussion 7 5 Conclusion 8 Ethics approval and consent to participate 8 Consent for publication 8 CRediT authorship contribution statement 8 Declaration of Competing Interest 8 Availability of data and materials 8 Acknowledgments 8 Appendix A Supporting information 8 References 8

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EN

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nonfiction