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Can I read Differential-Display Reverse Transcription-PCR (DDRT-PCR) (Springer Lab Manuals) on EtoBox?

Differential-Display Reverse Transcription-PCR (DDRT-PCR) (Springer Lab Manuals) by Dr. Sergio Colonna-Romano, Dr. Antonella Leone, Dr. Bruno Maresca (auth.) is a nonfiction available to read on EtoBox.

What is Differential-Display Reverse Transcription-PCR (DDRT-PCR) (Springer Lab Manuals) about?

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.<br> Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

Who reads Differential-Display Reverse Transcription-PCR (DDRT-PCR) (Springer Lab Manuals)?

It is typically read by self-directed learners exploring a subject in depth.

Common subject areas: history, science, philosophy, social sciences.

Author
Dr. Sergio Colonna-Romano, Dr. Antonella Leone, Dr. Bruno Maresca (auth.)
Publisher
Springer-Verlag Berlin Heidelberg
Published
1998
Language
EN
ISBN
9783540632979
Category
nonfiction
Subjects
Science, Chemistry, Medical

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