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Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals) by Dr. rer. nat. Thomas Köhler, Dirk Laßner, Prof. Dr. med. Harald Remke, Anne-Katrin Rost, Dr. rer. nat. Barbara Thamm, PD Dr. habil. Barbara Pustowoit (auth.) is a nonfiction available to read on EtoBox.

What is Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals) about?

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research.

Who reads Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals)?

Author

Dr. rer. nat. Thomas Köhler, Dirk Laßner, Prof. Dr. med. Harald Remke, Anne-Katrin Rost, Dr. rer. nat. Barbara Thamm, PD Dr. habil. Barbara Pustowoit (auth.)

Publisher

Springer-Verlag Berlin Heidelberg

Published

1996

Language

EN

ISBN

9783642797149

Category

nonfiction

Subjects

Medical, Science, Chemistry