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Enteroaggregative Escherichia coli induced activation of epidermal growth factor receptor contributes to IL-8 secretion by ... 1 1. Material & methods 2 1.1. Cell lines and culture conditions 2 1.2. Bacteria and infections 2 1.3. Inhibitor-treatment 2 1.4. Assessment of EAEC-induced activation of EGFR in human intestinal epithelial cells 2 1.5. Adherence assay 2 1.6. Fluorescence microscopy 2 1.7. Transcriptional profiling 3 1.8. Study on the expression of EAEC-induced activated downstream effectors of EGFR-mediated cell signaling pathways 5 1.9. IL-8 assay 5 1.10. Statistical analysis 5 2. Results 5 2.1. EAEC promotes activation of EGFR on human intestinal and colonic epithelial cells 5 2.2. EGFR is involved in stacked-brick adherence of EAEC to intestinal epithelial cells and EAEC-induced cytoskeletal rearrangem ... 5 2.3. Inhibition of EGFR activation attenuates EAEC-induced activated downstream effectors in intestinal epithelial cells 6 2.4. Downstream effectors and transcription factors are involved in EAEC-induced activated EGFR mediated IL-8 secretion from the ... 9 3. Discussion 9 Authors' contributions 14 Declaration of competing interest 14 Acknowledgements 14 Appendix A

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nonfiction