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Fluorescent probes for biomolecule detection under environmental stress 1 1 Introduction 3 2 Strategies for fluorescent probe design 4 2.1 Fluorescent signal transduction component 4 2.2 Fluorescence signal modulator moiety 5 2.2.1 Response unit 5 2.2.2 Fluorescence response mode 7 2.2.3 Fluorescent response mechanisms 7 2.3 Targeting unit 7 2.3.1 Organelle-targeting unit 8 2.3.1.1 Plasma membrane targeting 8 2.3.1.2 Mitochondrial targeting 8 2.3.1.3 Lysosome targeting 8 2.3.1.4 ER targeting 8 2.3.1.5 Golgi apparatus targeting 9 2.3.1.6 Nucleus targeting 9 2.3.2 Organ-targeting unit 9 2.3.3 Tumor-targeting unit 9 3 Applications of fluorescent probes in imaging environment-associated biomolecules 9 3.1 Fluorescence imaging under hypoxia stress 9 3.1.1 O2•− and H2Sn 10 3.1.2 Sulfane sulfur 10 3.1.3 H2Se 12 3.1.4 CO 12 3.1.5 Nitroreductase (NTR) 12 3.1.6 Cytochrome P450 (CYP450) reductase 12 3.1.7 Azoreductase 12 3.1.8 COX-2 13 3.1.9 L-xylosone 13 3.1.10 NTR + ATP 15 3.1.11 NTR + CYP450 reductase 15 3.1.12 NTR + Mitophagy 15 3.2 Fluorescence imaging during ischemia/reperfusion (I/R) 15 3.2.1 Superoxide anion (O2•−) 15 3.2.2 H2O2 15 3.2.3 NO 17 3.2.4 Peroxynitrite (ONOO–) 17 3.2.5 Glut

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nonfiction