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Bioimaging of superoxide anions in living cells and in vivo: Perfect visualization with fluorescence probes and their appli ... 1 1 Introduction 1 2 Fluorophores 2 2.1 Intrinsic fluorophores (natural state) 2 2.2 Extrinsic fluorophores (added state) 2 3 Responses of fluorescent probes 2 4 Properties of fluorescent probes 3 4.1 High selectivity 3 4.2 High sensitivity 3 4.3 Cytotoxicity 3 5 In vitro and In vivo detection of superoxide anion 3 5.1 Two-photon fluorescent probes 3 5.1.1 Targeting O2•─ 3 5.1.2 Targeting O2•─ associated with apoptosis, tumor and cancer cells 4 5.1.3 Targeting O2•─ associated with ER stress 7 5.1.4 Targeting O2•─ associated with diabetes 7 5.1.5 Targeting O2•─ associated with lysosome 7 5.1.6 Targeting O2•─ associated with liver injury 10 5.2 NIR fluorescent probes 10 5.2.1 Targeting O2•─ 11 5.2.2 Targeting O2•─ and H2Sn 14 5.2.3 Targeting O2•─ and H+ 14 5.3 Other ratiometric fluorescent probes 15 6 Conclusion and future perspective 19 Declaration of competing interest 19 Acknowledgements 19 References 19

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nonfiction