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Biomimetic polyphenol-coated nanoparticles by Co-assembly of mTOR inhibitor and photosensitizer for synergistic chemo-photo ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials 2 2.2 Cell culture 2 2.3 Preparation and characterization of nanoparticles 2 2.4 Stability of different nanoparticles in vitro 2 2.5 In vitro photothermal efficiency 3 2.6 Cellular uptake assay 3 2.7 ROS detection 3 2.8 In vitro cytotoxicity study 3 2.9 Cell cycle analysis 3 2.10 In vivo photothermal efficacy evaluation 3 2.11 In vivo tumor imaging 3 2.12 Evaluation of antitumor efficiency and histological analysis in vivo 3 2.13 Statistical analysis 4 3 Results and discussion 4 3.1 Preparation and characterization of IRE NPs 4 3.2 In vitro stability analysis of nanoparticles 4 3.3 Photothermal effect of IRE NPs 5 3.4 In vitro cellular uptake and ROS detection 6 3.5 In vitro cytotoxicity assay 6 3.6 Cell cycle analysis 7 3.7 In vivo fluorescence imaging 8 3.8 In vivo photothermal efficacy of IRE NPs 8 3.9 In vivo antitumor efficiency and safety evaluation 8 4 Conclusion 9 CRediT authorship contribution statement 10 Declaration of Competing Interest 10 Acknowledgements 10 Appendix A Supporting infor

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nonfiction