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One-pot self-assembly fabrication of chitosan coated hollow sphere for pH/ glutathione dual responsive drug delivery 1 1 Introduction 1 2 Material and methods 2 2.1 Materials and reagents 2 2.2 Preparation of chitosan-coated double layer hollow sphere 2 2.2.1 Preparation of removable unPMAA-NP core 2 2.2.2 Preparation of core/shell unPMAA/PMAA nanoparticles 2 2.2.3 Etching of uncrosslinked PMAA core 2 2.2.4 One-pot two-step process of DOX loading and chitosan coating 2 2.3 Preparation of DOX-loaded hollow sphere without chitosan 3 2.4 Determination of DOX loading capacity 3 2.5 In vitro controlled drug release 3 2.6 In vitro cytotoxicity 3 2.7 Analysis and characterization 3 3 Results and discussion 3 3.1 Preparation of drug-loaded particles 3 3.2 Study of drug loading behavior 4 3.3 In vitro drug release profile 5 3.4 In vitro cellular toxicity and intracellular distribution 6 4 Conclusion 7 CRediT authorship contribution statement 7 Declaration of Competing Interest 7 Data availability 7 Acknowledgements 7 Appendix A Supporting information 7 References 7
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- Language
- EN
- Category
- nonfiction
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