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Analyzing autophagosomes and mitophagosomes in the mouse brain using electron microscopy 2 Before you begin 2 Preparation one: fixative 2 Preparation two: 0.1 M cacodylate buffer 3 Preparation three: 0.3% lead citrate solution 3 Key resources table 3 Materials and equipment 4 Step-by-step method details 5 Animal perfusion and brain sectioning 5 Osmium tetroxide fixation, en bloc staining, dehydration, and resin infiltration 7 Embedding, isolation and mounting of the amygdala 8 Cutting ultrathin brain sections 8 Electron microscope imaging 9 Expected outcomes 11 Quantification and statistical analysis 11 Limitations 11 Troubleshooting 12 Problem 1 12 Potential solution 13 Problem 2 13 Potential solution 13 Problem 3 13 Potential solution 13 Problem 4 13 Potential solution 13 Problem 5 13 Potential solution 13 Resource availability 13 Lead contact 13 Materials availability 13 Data and code availability 14 Acknowledgments 14 Author contributions 14 Declaration of interests 14 References 14
Who reads Catalogue record?
It is typically read by self-directed learners exploring a subject in depth.
Common subject areas: history, science, philosophy, social sciences.
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