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Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells 2 Before you begin 2 Mice 2 Antibody panel preparation 2 Key resources table 3 Materials and equipment 4 5% FACS buffer 4 40× Liberase T-Flex stock 4 1× DNase stock 4 Epidermis digestion solution 5 Whole skin digestion solution 5 1× Foxp3 fixation/permeabilization working solution 5 1× permeabilization buffer working solution 5 Step-by-step method details 6 Skin collection 6 Generating cell suspension 6 Generating epidermal cell suspensions 6 Generating whole skin cell suspension 8 Staining 8 Data collection 10 Expected outcomes 10 Quantification and statistical analysis 12 Limitations 13 Troubleshooting 13 Problem 1 13 Potential solution 13 Problem 2 13 Potential solution 13 Problem 3 14 Potential solution 14 Problem 4 14 Potential solution 14 Problem 5 14 Potential solution 14 Resource availability 14 Lead contact 14 Materials availability 14 Data and code availability 14 Supplemental information 15 Acknowledgments 15 Author contributions 15 Declaration of interests 15 References 15

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Language

EN

Category

nonfiction