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Utilization of natural deep eutectic solvents and ultrasound-assisted extraction as green extraction technique for the reco ... 1 1 Introduction 1 2 Material and methods 2 2.1 Material 2 2.2 Date seed sample preparation 2 2.3 Preparation of NADES 2 2.4 Ultrasound-Assisted extraction (UAE) of date seed powder 2 2.5 Viscosity measurement 2 2.6 FTIR (Fourier-transform infrared) spectroscopy 2 2.7 Determination of total phenolic content (TPC) and total flavonoid content (TFC) 2 2.8 Determination of antioxidant activity 3 2.9 Response surface methodology for optimization of ultrasound-assisted extraction (UAE) of phenolic compounds from date s ... 3 2.10 Identification and quantification of the major phenolic compounds in date seed powder 3 2.11 Statistical analysis 3 3 Results and discussion 3 3.1 Viscosity of the synthesized NADES 3 3.2 FTIR spectrum of the synthesized NADES showing optimal extraction efficiency 4 3.3 Screening for the optimal NADESs for recovery of bioactive compounds from date seeds 5 3.4 Model fitting 6 3.5 Effect of the independent variables on the dependent variable (TPC) 6 3.6 Effect of the independent variables on DPPH radical scavenging activity 7 3.7 Verifica
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