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Combined effects of salinity and polystyrene microplastics exposure on the Pacific oysters Crassostrea gigas: Oxidative str ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Oyster collection and acclimatization 2 2.2 Tested MPs 2 2.3 Experimental design 2 2.4 Clearance rate of C. gigas 2 2.5 Determination of MPs uptake in oyster tissues 3 2.6 Determination of biochemical parameters 3 2.7 Determination of reactive oxygen species (ROS) production in C. gigas hemolymph 3 2.8 Gene expression analysis by quantitative real-time PCR 3 2.9 Integrated biomarker response (IBR) analysis 3 2.10 Untargeted metabolomics analysis 3 2.11 Data analysis 3 3 Results 3 3.1 MPs uptake in oyster tissues 3 3.2 Clearance rate of C. gigas 3 3.3 Stress biomarkers 4 3.4 ROS production of C. gigas hemolymph 4 3.5 mRNA expression of target genes 4 3.5.1 mRNA expression of stress-related genes 4 3.5.2 mRNA expression of energy-related genes 5 3.6 IBR analysis 6 3.7 Metabolomics analysis in gills 6 4 Discussion 6 4.1 MPs uptake in oysters and effects on clearance rate 6 4.2 Oxidative stress-related biomarkers 8 4.3 Stress-related genes 10 4.4 Energy metabolism and osmotic adjustment 10 4.5 IBR 10 5 Conclusio

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Language

UND

Category

nonfiction