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Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression 1 1. Introduction 1 2. Materials and methods 2 2.1. Cell culture 2 2.2. Cell synchronization 2 2.3. Immunofluorescence microscopy 2 2.4. Chromosome fractionation 2 2.5. Chromosome spreading 2 2.6. Live cell imaging 2 2.7. Fluorescence-activated cell sorting (FACS) 2 2.8. Western blot analysis 2 2.9. Chemicals and antibodies 3 2.10. In vitro Haspin kinase assay 3 2.11. Colony formation assay 3 2.12. Statistical analysis 3 3. Results 3 3.1. Ginsenoside Rg1 inhibits cancer cell proliferation through delaying mitotic exit 3 3.2. Ginsenoside Rg1 disrupts the integrity of mitotic chromosomes and spindle fibers 3 3.3. Ginsenoside Rg1 disturbs targeting of CPC to centromere 5 3.4. Ginsenoside Rg1 inhibits H3T3ph in a genetic background-dependent manner 5 4. Discussion 7 Declaration of competing interest 8 Acknowledgements 8 References 8
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