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Ginsenoside Rg1 protects mice against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced liver injury by inhibiting CYP1A1 through ... 1 1 Introduction 2 2 Materials and methods 2 2.1 Plant material 2 2.2 Cells 2 2.3 Animals 2 2.4 Experimental design 3 2.5 Serum biochemistry assays 3 2.6 Histopathological examination 3 2.7 Immunohistochemistry 3 2.8 RNA isolation and reverse transcription-quantitative polymerase chain reaction (qPCR) analysis 3 2.9 Western blot analysis 4 2.10 Immunofluorescence staining 4 2.11 Statistical analysis 4 3 Results 4 3.1 Effects of ginsenoside Rg1 on TCDD-induced AhR activation 4 3.2 Ginsenoside Rg1 alleviated liver injury in TCDD-treated mice 4 3.3 Ginsenoside Rg1 inhibited the TCDD-induced increase in CYP1A1 expression 5 3.4 Ginsenoside Rg1 inhibited TCDD-induced AhR activation 5 3.5 Ginsenoside Rg1 inhibited TCDD-induced AhR nuclear translocation 5 4 Discussion 5 5 Conclusions 10 Credit author statements 10 Funding 10 Declaration of competing interest 10 Acknowledgments 10 Appendix A Supplementary data 10 References 10

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UND

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nonfiction