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Foreword 0 Introduction 0 1 Scope 0 2 Normative references 0 3 Terms and definitions 0 4 Principle 0 5 Apparatus 0 6 Reagents 0 7 Preparation 0 7.1 Preparation of glassware 0 7.2 Preparation of solutions 0 7.3 Preparation of standards 0 7.3.1 General 0 7.3.2 Preparation of internal standard solutions 0 7.3.3 Preparation of calibration standard solutions 0 8 Sampling 0 9 Tobacco product preparation 0 10 Sample generation — Smoking of cigarettes 0 10.1 General 0 10.2 Linear smoking 0 10.3 Rotary smoking 0 11 Sample analysis 0 11.1 Preparation of sample 0 11.1.1 General 0 11.1.2 Extraction for linear smoking (44 mm pad) 0 11.1.3 Extraction for rotary smoking (92 mm pad) 0 11.1.4 Final sample preparation 0 11.2 Analysis of samples by LC-MS/MS 0 11.2.1 General 0 11.2.2 HPLC set-up parameters (Example) 0 11.2.3 Mobile phase (Example) 0 11.2.4 Mobile phase: Gradient (Example) 0 11.2.5 MS/MS Set-up parameters (Example) 0 11.3 Calculation 0 11.3.1 Calibration curve 0 11.3.2 Determination of the TSNAs concentrations 0 11.3.3 Sample quantification 0 11.3.4 Determination of mainstream smoke TSNA deliveries 0 12 Repeatability and reproducibility 0 13 Test report 0 Annex A (informative) Example

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