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Breeding of ‘DND358’: A new soybean cultivar for processing soy protein isolate with a hypocholesterolemic effect similar t ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Breeding materials 2 2.2 Breeding scheme 2 2.3 Analysis of the seed protein subunit composition 2 2.4 Amino acid assays 2 2.5 Isoflavone analysis 3 2.6 Defatted soy flour preparation 3 2.7 Preparation of DND358-SPI 3 2.8 Animals, diets, and experimental protocol 3 2.9 Blood collection and analysis 3 2.10 Liver and adipose tissue analysis 3 2.11 Statistical analysis 3 3 Results 3 3.1 Protein subunit composition of ‘DND358’ seeds and DND358-SPI 3 3.2 Agronomic performance of ‘DND358’ 4 3.3 Amino acid analysis 5 3.4 Isoflavone analysis 5 3.5 Effects of the ingestion of DND358-SPI on serum TC, TG, LDL-C, HDL-C levels and AI value 5 3.6 Effects of the ingestion of DND358-SPI on the histological characteristics of the liver and adipose tissue 7 3.7 Effects of the ingestion of DND358-SPI on liver TC, TG levels 7 4 Discussion 7 4.1 Subunit compositions and their association with the cholesterol-lowering effect 7 4.2 Amino acid compositions and their cholesterol-lowering effects 8 4.3 Isoflavone contents and their ch

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nonfiction