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Biochar-assisted degradation of oxytetracycline by Achromobacter denitrificans and underlying mechanisms 1 1 Introduction 1 2 Materials and methods 2 2.1 Chemicals and culture medium 2 2.2 Preparation of straw biochar 2 2.3 Isolation, identification and whole genome sequencing of oxytetracycline degrading strain 2 2.4 Identification of biodegradable products 2 2.5 Degradation of oxytetracycline in the degradation system 2 2.6 Determination of oxytetracycline concentration 3 2.7 Toxicity assessment 3 2.8 Metabolomic analysis 3 3 Results and discussion 4 3.1 Isolation of oxytetracycline degrading strain and whole genome analysis 4 3.1.1 Isolation and identification of degrading strain 4 3.1.2 Whole genome analysis of the strain 4 3.1.3 Analysis of biodegradable products 4 3.2 Biochar enhances the biodegradation of oxytetracycline 4 3.2.1 Adsorption characteristics and regeneration evaluation of biochar 4 3.2.2 Effect of biochar on degradation of oxytetracycline by the strain 4 3.2.3 Toxicity assessment of the degradation system 6 3.2.4 Effect of biochar on antioxidant enzyme activity 6 3.3 Metabolomics analysis of different degradation systems 7 3.3.1 Screening of differential metabo

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nonfiction