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Enhanced phenol biodegradation by Burkholderia PHL 5 with the assistant of nitrogen 1 1 Introduction 1 2 Materials and methods 2 2.1 Sample preparation, growth medium, and culture condition 2 2.2 Isolation and identification of phenol-degrading bacteria 2 2.3 Phenol biodegradation experiments 2 2.4 Characterizations 2 3 Results and discussion 3 3.1 Identification and characterization of strain PHL5 3 3.2 Effect of various factors on phenol degradation by strain PHL5 3 3.2.1 Oxygen contents 3 3.2.2 Electron acceptors 3 3.2.3 Initial phenol concentrations 3 3.2.4 Initial nitrate concentrations 4 3.2.5 Temperatures 4 3.2.6 Initial pH conditions 5 3.3 Optimization of environmental conditions by RSM 5 3.4 The growth profiles of strain PHL5 6 3.5 Analysis of 3D-EEM spectra during phenol degradation 7 3.6 Reusability of the strain PHL5 to degrade phenol 7 3.7 Phenol degradation mechanism of strain PHL5 7 3.7.1 LmPH and Nap gene expression of strain PHL5 7 3.7.2 Identification of intermediates in phenol degradation 7 4 Conclusion 8 Declaration of competing interest 9 Acknowledgements 9 Appendix A Supplementary data 9 References 9

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EN

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nonfiction