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Water deficit stress changes in physiological, biochemical and antioxidant characteristics of anise (Pimpinella anisum L.) 1 1 Introduction 1 2 Materials and methods 2 2.1 Experimental site 2 2.2 Trait measurements 2 2.2.1 CMS 3 2.2.2 Leaf chlorophyll content 3 2.2.3 Fv/Fm 3 2.2.4 Leaf temperature and stomatal conductance 3 2.2.5 Leaf relative water content (LRWC) 3 2.2.6 Oil extraction 3 2.2.6.1 Extraction of total lipids 3 2.2.6.2 Fatty acid methylation and analysis 3 2.2.7 Essential oil extraction 3 2.2.8 Gas chromatography analysis 3 2.2.9 Gas chromatography-mass spectrometry (GC-MS) 3 2.2.10 Identification of compounds 4 2.2.11 Extraction of polyphenol and analyses 4 2.2.11.1 Preparation of extracts 4 2.2.11.2 Total phenolic content (TPC) 4 2.2.11.3 RP-HPLC analysis of phenolic compounds 4 2.2.12 Antioxidant activity analysis 4 2.2.12.1 DPPH assay 4 2.2.12.2 Reducing power 4 2.2.12.3 Chelating effect on ferrous ions 4 2.3 Statistical analysis 4 3 Results 4 3.1 Water deficit effect on physiological and morphological parameters 4 3.2 Water deficit effect on TFA contents and proportions 5 3.3 Water deficit effect on EO content and components 6 3.4 Water deficit effect on TPC, TFC

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EN

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nonfiction