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Foreword 0 Introduction 0 1 Scope 0 2 Normative references 0 3 Terms and definitions 0 4 Principle 0 4.1 Virus extraction 0 4.2 RNA extraction 0 4.3 Real-time RT-PCR 0 4.4 Control materials 0 4.4.1 Process control virus 0 4.4.2 Double-stranded DNA (dsDNA) control 0 4.4.3 EC RNA control 0 4.5 Test results 0 5 Reagents 0 5.1 General 0 5.2 Reagents used as supplied 0 5.3 Prepared reagents 0 6 Equipment and consumables 0 7 Sampling 0 8 Procedure 0 8.1 General laboratory requirements 0 8.2 Virus extraction 0 8.2.1 Process control virus material 0 8.2.2 Negative process control 0 8.2.3 Food surfaces 0 8.2.4 Soft fruit, leaf, stem and bulb vegetables 0 8.2.5 Bottled water 0 8.2.6 Bivalve molluscan shellfish 0 8.3 RNA extraction 0 8.4 Real-time RT-PCR 0 8.4.1 General requirements 0 8.4.2 Real-time RT-PCR analysis 0 9 Interpretation of results 0 9.1 General 0 9.2 Construction of standard curves 0 9.3 Calculation of RT-PCR inhibition 0 9.4 Calculation of extraction efficiency 0 9.5 Sample quantification 0 10 Expression of results 0 11 Precision 0 11.1 Interlaboratory study 0 11.2 Repeatability 0 11.3 Reproducibility limit 0 12 Test report 0 Annex A (normative) Diagram of procedure 0 Annex B

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