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Improving tyrosinase inhibitory activity of grass carp fish scale gelatin hydrolysate by gastrointestinal digestion: Purifi ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials 2 2.2 In vitro digestion 3 2.3 Analysis of the molecular weight (MW) distribution of TYIPs-GI 3 2.4 Screening and identification of tyrosinase inhibitory peptides in TYIPS-GI 3 2.5 Enzyme assay 3 2.5.1 Inhibition on tyrosinase activity 3 2.5.2 Inhibition mechanism and type analysis on tyrosinase 4 2.6 Molecular docking 4 2.7 Determination of whitening activity by zebrafish 5 2.7.1 Maximum tolerated concentration determination (MTC) 5 2.7.2 Determination of whitening efficacy 5 2.7.3 Determination of tyrosinase activity inhibition ability 5 2.7.4 Determination of melanin inhibition ability 6 2.8 Determination of skin lightning effect by zebrafish 6 2.8.1 Maximum tolerated concentration determination (MTC) 6 2.8.2 Determination of skin lightning 6 2.9 Statistical analysis 7 3 Results and discussion 7 3.1 Tyrosinase inhibitory activity and MW distribution before and after simulated GI digestion of TYIPs 7 3.2 Identification and evaluation of tyrosinase inhibitory activity of synthetic peptides 8 3.3 I

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UND

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nonfiction