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In-depth characterization revealed polymer type and chemical content specific effects of microplastic on Dreissena bugensis 1 1 Introduction 2 2 Materials and methods 2 2.1 MP and control particles preparation 2 2.2 MP and control particle characterization 3 2.2.1 Size and shape distribution 3 2.2.2 Chemical composition of MP 3 2.2.3 Distribution and behavior of particles in the water column 3 2.3 Mussel collection 4 2.4 Experimental setup 4 2.5 Behavior monitoring 4 2.5.1 Voltage output– Mussel behavior adjustment 4 2.5.2 Behavior monitoring during particle exposure 5 2.6 Biochemical assays 5 2.7 Uptake of MP into the mussels 6 2.8 Statistical analysis 6 2.9 Proteomic analysis 6 2.10 Proteomic data processing 6 3 Results 6 3.1 MP characterization 6 3.1.1 LDV measurements – Suspension of particles in the water column 7 3.2 Ingestion of MP 8 3.3 Behavioral endpoints 8 3.4 Biochemical alterations 9 3.5 Proteomic analysis 9 4 Discussion 10 4.1 MP properties 10 4.2 Mussels are not able to distinguish between natural and artificial particulate matter 11 4.3 Mussels are "blind" to MP, but are affected by it – depending on the polymer type 12 4.4 Polymer type dependent effects on the dige

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nonfiction