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Molecular, biochemical, and comparative genome analysis of a rhizobacterial strain Klebsiella Sp. KBG6.2 imparting salt str ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Soil sample and plant material 2 2.2 Isolation and cultivation of bacteria strains 2 2.3 Characterization of bacterial strain 2 2.4 Effect of abiotic stress on the growth of PGPR 2 2.5 Whole-genome sequencing and analysis 2 2.6 Phylogenetic analysis 3 2.7 Comparative genome analysis 3 2.8 Comparative PGPR functional genes analysis 3 2.9 Effect of KBG6.2 strain in imparting salt tolerance to rice seedlings 3 2.9.1 Experimental plant growth design 3 2.9.2 Bacterial inoculation 3 2.10 Determination of plant growth parameters on the application of PGPR 3 2.10.1 Morphological parameters: germination percentage, shoot, root length, and biomass measurement 3 2.10.2 Estimation of biochemical parameters 3 2.10.2.1 Chlorophyll estimation 3 2.10.2.2 Reducing sugar estimation 3 2.10.2.3 Proline and malondialdehyde (MDA) estimation 3 2.10.2.4 Estimation of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activity 4 2.11 RNA extraction, cDNA synthesis, and real-time PCR (qRT-PCR) analysis 4 2.12 Root colon

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nonfiction