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The potential role of plasma membrane proteins in response to Zn stress in rice roots based on iTRAQ and PRM under low Cd c ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Plant materials and Zn/Cd treatment 2 2.2 Protein extraction, digestion, and peptide quantification 3 2.2.1 Extraction of PM proteins from rice samples 3 2.2.2 Protein enzymatic hydrolysis, digestion, and peptide quantification 3 2.3 iTRAQ labeling and peptide fractionation with strong cation exchange (SCX) 3 2.4 Liquid chromatography mass spectrometry (LC-MS/MS) analysis 3 2.5 Identification and quantitative analysis of PM proteins 3 2.6 Bioinformatics analysis 3 2.7 Protein-Protein Interact Network (PPI) 4 2.8 Parallel reaction monitoring (PRM) analysis 4 3 Results and discussions 4 3.1 Effect of Zn and Cd on physiology performance of rice seedling 4 3.2 Effect of Zn on Zn and Cd accumulation in rice seedlings treated with low Cd 4 3.3 Overview of quantitative proteomics in response to Zn stress 5 3.4 Hierarchical clustering analysis of the DEPs 5 3.5 Identification and classification of the DEPs 7 3.6 KEGG pathway enrichment of the DEPs 8 3.7 Proteins associated with Zn2+ and Cd2+ transport in rice roots
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It is typically read by self-directed learners exploring a subject in depth.
Common subject areas: history, science, philosophy, social sciences.
- Language
- EN
- Category
- nonfiction
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