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Protein structure in model infant milk formulas impacts their kinetics of hydrolysis under in vitro dynamic digestion 1 1 Introduction 1 2 Material and methods 2 2.1 Materials 2 2.1.1 Infant milk formula ingredients 2 2.1.2 In vitro digestion material 2 2.2 Preparation of the infant milk formulas 2 2.3 In vitro digestion protocol 2 2.4 Digesta microstructure characterization 3 2.4.1 Particle size distribution 3 2.4.2 Confocal laser scanning microscopy 3 2.5 Proteolysis kinetics 3 2.5.1 SDS-PAGE and intact protein semi-quantification 3 2.5.2 OPA assay and degree of hydrolysis 3 2.6 In vitro protein digestibility 3 2.7 Statistical analysis 3 2.8 Peptidomic analysis 4 2.8.1 Peptide identification and quantification 4 2.8.2 Peptidomic data analysis 4 3 Results and discussion 4 3.1 Structural changes during in vitro gastric digestion 4 3.2 Protein hydrolysis during in vitro gastrointestinal digestion 5 3.2.1 Residual intact proteins 5 3.2.2 Degree of hydrolysis and protein digestibility 7 3.3 Peptide profile during in vitro gastrointestinal digestion 7 3.3.1 Peptide identification 8 3.3.2 Peptide clustering and kinetics of peptide release 8 3.3.3 Peptidomic profiles of the major protein

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nonfiction