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Efficient hydroxylamine removal through heterotrophic nitrification by novel bacterium Glutamicibacter arilaitensis EM-H8 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials 2 2.2 Culture media 2 2.3 Enrichment and isolation of bacteria 2 2.4 Screening bacteria that efficiently remove hydroxylamine 2 2.5 Identification of bacteria 3 2.6 Analysis of the influence of environmental conditions on the hydroxylamine removal ability of strain EM-H8 3 2.7 Nitrogen balance analysis 3 2.8 Enzyme assay 3 2.9 Analytical methods 3 3 Results and discussion 3 3.1 Enrichment, isolation, and purification of bacteria exhibiting high hydroxylamine removal 3 3.2 Identification of high hydroxylamine removal capacity of bacterium 3 3.3 Hydroxylamine removal performance of strain EM-H8 under different conditions 4 3.3.1 Effect of different carbon sources on hydroxylamine removal 4 3.3.2 Selection of optimal C/N ratio 4 3.3.3 Screening of optimum pH range for strain EM-H8 7 3.3.4 Optimal dissolved oxygen concentration strain EM-H8 7 3.3.5 Selection of optimum inoculum size for hydroxylamine removal 7 3.3.6 Optimum temperature for strain EM-H8 7 3.4 Analysis of hydroxylamine removal pathway 7 3.5 En

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nonfiction