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Can I read Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration: IMPACT OF miR-155 IN INTERVERTEBRAL DISC DEGENERATION on EtoBox?
Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration: IMPACT OF miR-155 IN INTERVERTEBRAL DISC DEGENERATION by Wei-Lin Zhang; Yu-Fei Chen; Hong-Zheng Meng; Jun-Jie Du; Guan-Nan Luan; Hai-Qiang Wang; Mao-Wei Yang; Zhuo-Jing Luo is a Medicine article available to read on EtoBox.
What is Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration: IMPACT OF miR-155 IN INTERVERTEBRAL DISC DEGENERATION about?
The molecular mechanisms of intervertebral disc degeneration (IDD) remain elusive. We found that miR-155 is down-regulated in degenerative nucleus pulposus (NP), and more severe degeneration is correlated with higher matrix metallopeptidase 16 (MMP-16) expression. MMP-16 also degraded matrix aggrecan. Here, we addressed the in vivo miR-155-mediated pathological impact on IDD using a classic puncture mouse model. Lentiviral upregulated-miR-155 or downregulated-miR-155 was transduced into the discs of C57 mice, which was validated by real-time polymerase chain reaction (real-time PCR) and in situ hybridization. Immunohistochemistry and western blotting revealed that up-regulation of miR-155 resulted in down-regulation of MMP-16 and an increase in aggrecan and collagen type II in mouse NP; whereas, down-regulation of miR-155 resulted in up-regulation of MMP-16 and a decrease in aggrecan in mouse NP. Radiographic and histological analysis showed that the up-regulation of miR-155 attenuated IDD, while down-regulation of miR-155 resulted in the deterioration of IDD. These findings indicate that decreased miR-155 contributed to the up-regulation of MMP-16 in vivo, and MMP-16 further degra
Who reads Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration: IMPACT OF miR-155 IN INTERVERTEBRAL DISC DEGENERATION?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Wei-Lin Zhang; Yu-Fei Chen; Hong-Zheng Meng; Jun-Jie Du; Guan-Nan Luan; Hai-Qiang Wang; Mao-Wei Yang; Zhuo-Jing Luo
- Publisher
- Wiley
- Published
- 2016
- Language
- EN
- Field
- Medicine (Health Sciences)